A landscape of checkpoint blockade resistance in cancer: underlying mechanisms and current strategies to overcome resistance

ABSTRACT The discovery of immune checkpoints and the development of immune checkpoint inhibitors (ICI) have achieved a durable response in advanced-stage cancer patients. However, there is still a high proportion of patients who do not benefit from ICI therapy due to a lack of response when first treated (primary resistance) or detection of disease progression months after objective response is observed (acquired resistance). Here, we review the current FDA-approved ICI for the treatment of certain solid malignancies, evaluate the contrasting responses to checkpoint blockade in different cancer types, explore the known mechanisms associated with checkpoint blockade resistance (CBR), and assess current strategies in the field that seek to overcome these mechanisms. In order to improve current therapies and develop new ones, the immunotherapy field still has an unmet need in identifying other molecules that act as immune checkpoints, and uncovering other mechanisms that promote CBR.


Introduction
3][4][5] Under homeostasis conditions, the role of immune checkpoints, along with costimulatory signals, is to maintain self-tolerance and regulate the type and magnitude of the immune response. 2However, tumor cells can dysregulate the signaling of immune checkpoints to allow the evasion of tumor cells. 3Based on the immense role of checkpoint signaling in cancer, targeting checkpoint molecules is a rational strategy to promote anti-tumor immunity and, indeed, several immune checkpoint inhibitors (ICI) have been developed and proven effective in the clinical setting. 1,4TLA-4 is an immunoglobulin-related receptor that is upregulated on conventional T cells following activation. 5,6s a homolog of CD28, it also binds CD80 and CD86 expressed on antigen-presenting cells (APCs) but at a higher affinity, effectively competing with CD28 for ligand binding.In contrast to CD28, CTLA-4 attenuates T cell responses potentially through various reported mechanisms such as interfering with molecules downstream of CD28 and the T cell receptor (TCR). 7,8In addition, CTLA-4 is highly expressed on regulatory T cells (Tregs) and is believed to be important in Treg homeostasis and function. 91][12] The approval of ipilimumab was based on the results of the MDX010-020 clinical trial (NCT00094653) showing that among 676 randomized patients, 20% and 16% of the patients survived ≥2 years and ≥3 years, respectively. 4This was a significant improvement in overall survival (OS) over chemotherapy and cytokine-based therapies, which were used as standard-of-care (SOC) treatment for this patient population. 13Currently, ipililumab in combination with nivolumab is approved for colorectal cancer (CRC), hepatocellular carcinoma (HCC), mesothelioma, and non-small cell lung cancer [NSCLC]. 2,14For melanoma, ipilimumab is approved as monotherapy or in combination with nivolumab.
The discovery of PD-1-a receptor expressed on activated T cells and its ligand PD-L1, expressed on tumor cells -identified another immunosuppressive mechanism that inactivates TCR and/or CD28 signaling, thereby impairing the normal function of cytotoxic effector T cells (Teff).Nivolumab was the first anti-PD-1 blocking antibody that was approved for treatment of inoperable or metastatic melanoma based on the CheckMate 037 (NCT01721746) clinical trial. 15The trial demonstrated that a greater proportion of advanced melanoma patients achieved an objective response and fewer toxic effects compared to the alternative chemotherapeutic regimen. 15Since then, other anti-PD-1 therapies such as pembrolizumab, cemiplimab, and dostarlimab have also been approved. 2Targeting PD-L1 is also a viable approach to block the PD-1/PD-L1 inhibition pathway.Due to the crosstalk between the MAPK and the PI3K signaling pathways, an oncogenic mutation in MAPK pathway or a loss of PTEN expression can cause the enhancement of PI3K-AKT signaling as well.Similarly, a mutation or silencing of β-catenin or tumor suppressor wnt protein, results in dysregulation of the WNT/β-catenin pathway, promoting aberrant signaling of b -catenin contributing to the absence of T cell expression signature.Mutations in the IFN-g receptor and defects on the IRF1 or JAK1/2 genes can contribute to T cell desensitization and consequently promote acquired resistance to ICIs.Specifically, mutations of PD-L1, PD-L2, and JAK2 genes resulting from the amplification of the locus that contains these genes promote the formation of the PD-1/TCR inhibitory microcluster, which results in the inhibition of T cell activation.(b) Another resistance mechanism is developed after defects in the APM specifically due to mutations of B2M and TAP proteins.These mutations make the antigen unable to reach the tumor cell's surface and be recognized and cleared out by CD8 + T cells.(c) the interaction between T cells, tumor cells, APCs, and immunosuppressive cells (MDSCs) throughout immune checkpoints such as VISTA, LAG-3, TIGIT, and TIM-3, triggers and inhibitory signal causing the exhaustion of T cells, blockage of TCR signaling, and decreasing T cell activation and TCR expression.The JAVELIN Merkel 200 clinical trial (NCT02155647) paved the way for the approval of the PD-L1 blocking antibody, avelumab, for the treatment of metastatic Merkel carcinoma (MCC). 12,16In this study, 31.8%(28 out of 88) of MCC patients whose disease had progressed on or after chemotherapy experienced objective responses with avelumab treatment. 16Avelumab was also later approved for patients with locally advanced or metastatic urothelial carcinoma (mUC). 12,17Durvalumab and atezolizumab are among the antibodies blocking PD-L1 that have been FDA-approved for the treatment of patients with locally advanced mUC. 12,18ollectively, ICIs targeting the PD-1/PD-L1 axis are therapeutic options for basal cell carcinoma (BCC), cutaneous squamous cell carcinoma (CSCC), NSCLC, CRC, HCC, Hodgkin lymphoma (HL), head and neck squamous cell carcinoma (HNSCC), mesothelioma, renal cell carcinoma (RCC), breast cancer (BC), large B cell lymphoma, and endometrial, esophageal, and gastric carcinomas. 2 The blockade of multiple checkpoints as a combinatorial therapy has improved the immunomodulatory ability of the immune system in achieving tumor cell clearance.The combination of ipilimumab plus nivolumab has also been FDAapproved for the treatment of advanced unresectable/metastatic melanoma cancer patients, after showing an improvement in overall response rate (ORR), progression-free survival (PFS), and OS against the SOC chemotherapy (sunitinib). 12,15,19,20urthermore, the combination has resulted in improved PFS when compared to ipilimumab alone in melanoma. 20In fact, except for melanoma, approved indications of ipilimumab are usually in combination with nivolumab. 2espite the durable response observed with immune checkpoint blockade (ICB) as part of the SOC for the treatment of patients with a broad range of advanced-stage cancers, only a limited population of patients benefit from ICI therapy. 1,21,22he lack of response to ICI, also called checkpoint blockade resistance (CBR), can be categorized as primary or acquired. 1,22,23Primary CBR is observed in patients who do not respond to initial treatment, while acquired CBR is defined as confirmed early response to the therapy, but with eventual disease progression after prolonged treatment. 1,22,23For example, primary CBR is observed in metastatic BC patients, 24,25 in about 45-70% of melanoma patients, 19,26 and 7-27% of NSCLC patients. 27,28[38][39][40] In this review, we will discuss the response of ICI in the real world and how this compares to the responses observed in clinical trials, as well as the underlying mechanisms promoting primary and acquired CBR.Finally, we aim to discuss current efforts to suppress these mechanisms that lead to CBR with the end goal of improving patient response to ICI therapy.

Contrasting responses to immune checkpoint blockade in the real-world
The response of patients treated with ICIs in the "real world" occasionally looks different from the responses observed and reported by controlled clinical trials run in different institutions.While there are data showing the consistency between the PFS and objective response rate observed in ICI-treated patients in the real world and the responses reported in the clinical trials, [41][42][43] other studies have highlighted a gap between the outcomes of immunotherapy reported by clinical trials (efficacy) and the outcomes observed in real-world (effectiveness) across several cancer types. 14,44For example, data support the groundbreaking efficacy and durable response achieved by ICIs for the treatment of advanced-stage cancers, specifically in melanoma, 45,46 advanced MCC, 47,48 RCC, 49 and NSCLC. 49nterestingly, these cancers have in common a high density of immune subsets infiltrated in the tumor microenvironment (TME) and the accumulation of pro-inflammatory cytokines that make them classified as "hot" tumors. 49,50hese characteristics, among other factors that will be discussed later in the review, improve the response rate to ICI therapy in "hot" tumors. 50Specifically, in melanoma patients, median survival improved from 6 months when patients were treated with cytotoxic agents such as temozolomide to 6.5 years following treatment with anti-CTLA -4, anti-PD1 or a combination of both ICIs. 51A study of 60 patients with desmoplastic melanoma treated with antibodies blocking PD-1 or PD-L1 showed an objective response of 70% and 32% of them showed a complete response (CR). 45Another study of 230 metastatic melanoma patients showed a longer PFS using a combination of anti-CTLA-4 and anti-PD-1, 46 highlighting the benefits of blocking different immune checkpoints to improve the immune response.However, it is important to mention that twothirds of melanoma patients failed to achieve complete response after ICI therapy, hence the challenge of primary and acquired resistance faced by these patients. 513][54][55] Indeed, data from retrospective analyses confirmed the optimistic overview to continue using ICIs for the treatment of metastatic MCC patients. 52,56This study evaluated patients from two large academic medical centers who were treated with alternative ICIs after progressive disease (PD).Anti-CTLA-4 alone or in combination with anti-PD-1 revealed an overall response (OR) in 31% of the patients; one patient with MCC refractory -anti-PD-1 and anti-CTLA-4-had tumor regression with anti-PD-L1. 52It is worth mentioning that this study is the largest case series to date describing the anti-tumor efficacy of anti-PD-L1 as a secondline treatment for MCC patients with anti-PD-1 refractory disease.(e) physical barriers also prevent the infiltration of effector T cells in the TME, and tumor vasculature represents one of these barriers.Therefore, VEGF targeting using small molecule inhibitors or mAbs represents an effective strategy to disrupt angiogenic pathways that are fostering the aberrant vasculature.(f) another physical barrier is the extracellular matrix that is composed mainly of collagen.Collagen is produced by TAMs, CAFs, and tumor cells and can impair immune activity through interactions with LAIR-1.Blockade of LAIR-1 combined with PD-1, and TGF-β blockade can increase M1 TAMs population, CD8 + T cell infiltration, while decreasing TGF-β and collagen.Figure adapted from J Clin Invest.2022;132 8 : e155148.https://doi.Org/10.1172/JCI155148.lastly, repolarization of the immunosuppressive microenvironment as an alternative strategy to overcome certain resistant mechanisms can be achieved by (g) the use of mAbs targeting CD33 and TRAIL-R2 receptors expressed in tumor cells were demonstrated to eliminate MDSCs.The use of anti-CFR-1 were demonstrated to eliminate M2 macrophages.Likewise, the depletion of tregs using anti-CD4, anti-CD25 or anti-GITR in combination with ICI have shown to improve CD8+ T cell activity.Another approach uses to decrease the NSCLC, as one of the most common and deadliest malignancies worldwide, also benefits from blocking the immune checkpoints PD-1, PD-L1, and CTLA-4 alone or in combination with SOC. 29 Approximately 20-30% of NSCLC patients respond to ICI treatment and several studies continue to evaluate combination therapies to improve the response rate. 29Despite the progress observed, response to ICI is not always compared equally to the response reported by clinical trials.For example, a retrospective analysis of stage-IV NSCLC patients treated with pembrolizumab as a first-line treatment and nivolumab as a second-line treatment showed that the OS in real world was significantly shorter in patients receiving pembrolizumab as a first-line treatment compared to ORR as reported in clinical trials. 14he PFS of patients in pembrolizumab or nivolumab cohorts was comparable between real-world and trials. 14Likewise, another retrospective study with a cohort of 19,529 Medicare patients with NSCLC, ranging from 66 to 89 years old, showed an unadjusted median survival of 11.4 months among patients receiving single-agent pembrolizumab, which was 15 months shorter than the median survival of pembrolizumab-treated participants in the KEYNOTE-024 trial. 44,57In many studies, the use of chemoimmunotherapy for cancer patients with advanced stages has been incorporated into their SOC treatment regimen [58][59][60][61] ; however, this study also showed that the unadjusted median survival for patients receiving chemoimmunotherapy (platinum/pemetrexed/pembrolizumab) was 12.9 months, approximately 10 months shorter than for participants in the KEYNOTE-189 trial who received the same regimen 44,62 In total contrast to the response observed in "hot" tumors toward ICIs, the response documented in "cold" tumors is poor. 49"Cold" tumors such as prostate, pancreatic, and most colorectal cancers are characterized for being non-T cell infiltrated (non-inflamed) and largely resistant to ICI therapy. 49,50As of today, the only ICI FDA-approved treatment for prostate cancer patients is pembrolizumab (anti-PD-1), but only for metastatic castration-resistant prostate cancer (mCRPC) patients with high tumor mutational burden (TMB-H), high microsatellite instability (MSI-H), or mismatch repair deficiency (MMR-D). 63However, several clinical trials continue to show the limited response of mCRPC patients to singleagent ICI therapy, including pembrolizumab and other ICIs such as atezolizumab (anti-PD-L1) and ipilimumab (anti-CTLA-4). 63Besides poor infiltration in "cold tumors," these tumors rarely express PD-L1 (immunologically ignorant) and show low expression of neoantigens and immunosuppressive TME, all contributing factors to their unresponsiveness to checkpoint blockade. 49

Checkpoint blockade resistance
CBR needs to be discussed not only in terms of the biological concepts, but also in the complex scenario of the clinical setting, making it challenging to convey a uniform clinical definition of resistance to ICB for advanced diseases. 23To bring consensus to defining CBR, several principles and guidelines have to be considered and established, including the underlying mechanisms driving primary or acquired resistance, [64][65][66] treatment duration criteria to determine the cutoff to distinguish primary resistance, 23,66 and treatment discontinuation criteria to determine if acquired resistance is considered even after cessation of the ICI. 23,66In response to this heterogenous scenario, the Society for Immunotherapy of Cancer (SITC) taskforce agreed to take into consideration the duration of drug exposure, scan requirements, and response evaluation to define both primary and acquired resistance.
First, the taskforce stated that it was critical to define a minimal exposure timeframe for patients treated with an FDA-approved PD-1 or PD-L1 inhibitor to derive any possible clinical benefit. 23The taskforce established a required exposure to ICI therapy of at least 6 weeks but not more than 6 months.For patients with indolent tumor types, however, this timeframe needs to be redefined. 23,67To determine whether a treated patient is showing immune checkpoint primary resistance, a confirmatory scan needs to be performed to validate PD. 23 On the other hand, the taskforce disagreed on which response evaluation criteria -Response Evaluation Criteria in Solid Tumors 68 or RECIST 1.1-was to be employed and agreed that immune-based Response Evaluation Criteria in Solid Tumors (iRECIST) could not be used as a sole criterion to determine ICI efficacy.Although iRECIST was created to address cases of mixed responses and pseudoprogression, the taskforce stated that further validation is needed. 23,68However, the group agreed that the use of a fluorodeoxyglucose (FDG)/positron emission tomography (PET) scan combined with metabolically active immune infiltrates may be used as an indicator of response to ICI therapy. 23he taskforce defined acquired resistance in advanced disease settings, otherwise known as secondary resistance, when a patient is treated for 6 months or longer, has a CR, partial response (PR), or prolonged stable disease (SD) -confirmed by scan -for more than 6 months and then presents PD in the setting of ongoing treatment. 23Members of the taskforce did not reach a consensus regarding the requirement of confirmatory imaging for validating secondary resistance.Two of the immunosuppressive population that abrogates the effect of ICIs, is the use of small molecule inhibitors targeting CXCR1/2 which are receptors to chemokines essential for the recruitment of MDSCs and TANs.Growing evidence have shown that blocking TGF-b and PD-L1 simultaneously can decrease immunosuppressive population in the TME.(h) the use of inhibitors against IDO enzyme, halt the conversion of tryptophan to kynurenine allowing the increase of NK cells and CD8 + T cell and the decrease of Tregs.ICD: immunogenic cell death; DAMPs: damage-associated molecular patterns; APC: antigen presenting cells; TME: tumor microenvironment; ACT: adoptive cell transfer; TCR T: T cell receptor-engineered T cells; CAR T cells: chimeric antigen receptor T cells; MCH: major histocompatibility complex; VEGF: vascular endothelial growth factor; TAMs: tumor-associated macrophages; LAIR-1: leukocyte-associated immunoglobulin-like receptor-1; CAFs: cancer-associated fibroblasts; TANs: tumor-associated neutrophils; PD-1: program cell-death 1; TGF-β:transforming growth factor beta.
three groups recommended a scan within 4-12 weeks after evidence of PD, including verification of ≥2 metastatic sites/ lesions for patients with multiple metastases, for acquired resistance confirmation. 23ncommon response patterns represent an additional challenge in defining clinical resistance to ICIs. 23,69One of these patterns is known as pseudoprogression and describes patients who appear to have PD after radiographic confirmation, but experienced tumor shrinkage months after treatment cessation. 23,69,70This type of response was first observed in a phase II clinical trial that evaluated the efficacy of ipilimumab in metastatic melanoma patients. 71In this study, Wolchok et al. showed that 9.7% (22 out of 227) of the treated patients were characterized with PD -following World Health Organization (WHO) and iRECIST guidelines -in spite of having clinical responses (PR and SD). 70,71Clinical trial protocols did not require patient follow-up after PD was observed, thus limiting data collection for those showing a response after leaving the clinical trial.Accordingly, the number of patients with pseudoprogression may have been underestimated. 71urrent data support that pseudoprogression occurs in approximately 5-10% of the patients receiving anti-CTLA-4 or anti-PD-L1 therapy, across several solid tumor types. 23,70,72uilding on these findings, a novel benchmark designated as immune-related response criteria (irRC) is currently used to differentiate pseudoprogression from true progression and may provide a more comprehensive evaluation of the response toward the immuno-oncology (IO) agent. 71,72As such, a study evaluating irRC and RECIST criteria in advanced melanoma patients treated with pembrolizumab showed that the sole use of RECIST criteria may have underestimated the benefit of the checkpoint in approximately 15% of the patients. 73

T cells exclusion and dysfunction in the TME
The density of preexisting T cells in the TME as well as their functionality are two of the factors used to determine the outcome of ICI therapy. 1 Immune-excluded tumors -"cold tumors" -lack infiltrating effector T cells and are less prone to respond to ICI therapy.This lack of response may be due to primary resistance conferred by mutations and dysregulation of cell signaling pathways in tumor cells, resulting in poor T cell recruitment and T cell dysfunction. 1,39For example, a study in highly aggressive HCC determined that low T cell numbers may be associated with deletions in the MAX/TP53 genes, which in turn resulted in the downregulation of TP53related chemokines that are pivotal in T cell recruitment. 74,75ultiple oncogenic signaling pathways affecting primary resistance have been identified, including mitogenactivated protein kinase (MAPK)/extracellular signalregulated kinase 76 signaling pathways and/or loss of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression. 1,74,77Dysregulated MAPK/ERK pathways induce the production of vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8), resulting in an inhibitory effect on the recruitment of T cells and defects in T-cell activation and differentiation (Figure 1a). 74,78fferent cancer types harbor somatic mutations related to the MAPK pathways, many of which had been demonstrated to be oncogenic. 79For example, approximately 18% of HNSCC patients harbor MAPK pathway mutations and half of these mutations are oncogenic in nature. 79,80As a result of the crosstalk between the MAPK and the phosphoinositide-3-kinase -protein kinase B/Akt (PI3K-AKT) signaling pathways, an oncogenic mutation in MAPK pathway -or loss of PTEN expression -will cause the enhancement of PI3K-AKT signaling, associated with resistance to checkpoint blockade resistance (Figure 1a). 1,77,81A study of melanoma cancer patients correlated the constitutive activation of PI3K-AKT signaling, due to PTEN loss, with low numbers and impaired function of the tumor-infiltrated lymphocytes (TILs), as well as poor outcomes after PD-1 inhibitor treatment. 81nother common mutation in cancer occurs in the WNT/β-catenin signaling pathway, particularly due to mutation or silencing of the Wnt protein, which acts as a tumor suppressor (Figure 1a). 82In melanoma cancer patients, dysregulation of the WNT/β-catenin pathway has been correlated with unresponsiveness to ICI therapy. 82This was corroborated in a murine melanoma model wherein continuous β-catenin signaling in the TME contributed to the absence of T cell expression signature and, consequently, resistance to anti-PD-L1 and anti-CTLA -4 therapy. 82The growing evidence of the relationship between the activation of WNT/β-catenin signaling and ICI resistance has given the rationale to target β-catenin pathway to enhance the efficacy of ICIs. 83ancer-associated resistance also arises as a result of mutations in the IFN-γ signaling pathway (Figure 1a). 1,77FN-γ is a pleiotropic cytokine with antitumor and immunomodulatory functions; thus, it plays an important role in both innate and adaptive immune responses. 84IFN-γ activates its receptor (IFNG1/2) which is intracellularly associated with kinases from the Janus kinase (JAK) family (JAK1 and JAK2), inducing the expression of genes involved in cell cycle regulation, apoptosis, growth inhibition, and tumor suppression. 84The growing data support the idea that defects in the IFN-γ signaling pathway cause T cell desensitization, allowing acquired resistance to checkpoint blockade. 1,77,85,86Recent data showed that biopsies from melanoma patients who did not respond to anti-CTLA-4 therapy have mutations in IFNG1/2, JAK2, and interferon regulatory factor 1 (IRF1) genes. 85In a separate study, two out of four metastatic melanoma patients who had progressive disease after initial objective tumor regression with pembrolizumab had loss-of-function mutations in JAK1 or JAK2 genes. 86Mutations in the IFN-γ signaling pathway can also foster primary or acquired resistance via constitutive PD-L1 expression or the loss of PD-L1 expression in cancer cells. 1,77The mutations in the first scenario arise after the amplification of the locus in chromosome 9 that contains the genes for PD-L1, PD-L2, and JAK2. 1 The interaction of the PD-1 receptor with its ligand, PD-L1, now overexpressed in tumor cells due to the mutation, forms the PD-1/TCR inhibitory microcluster, resulting in the inhibition of T cell activation (Figure 1a). 1 In the second scenario, in which PD-L1 expression is no longer inducible by IFN-γ, anti-PD-1/PD-L1 antibodies would not be effective, and patients would manifest primary resistance to ICI. 1,87

Defects in antigen processing machinery and lack of tumor-associated antigens
The antigen processing and presentation pathway are critical in the immunosurveillance of cancer. 88,89Tumor antigen recognition by CD8 + T cells requires that for an effector CD8 + T cell to recognize an antigen from a tumor cell, the antigen must be expressed in the surface of the tumor cells and the T cell must be able to recognize that antigen presented by an MHC class I molecule (MHCI). 89In humans, MHCI molecules are heterodimers composed of heavy chains encoded by Human Leukocyte Antigen (HLA) genes (HLA-A, HLA-B, and HLA-C) and a light chain b 2 -microglobulin (B2M). 88,89ntigen processing is a multi-step procedure that includes antigen fragmentation by the proteosome, relocation of the antigenic peptide to the endoplasmic reticulum (ER) by specific transporter proteins, docking of the peptide on an MHC-I molecule, and the transport of the MHC-I-peptide complex to the cell surface. 89vidence indicates that mutations and epigenetic modifications in the cancer DNA that alter the antigen processing and presentation machinery can contribute to checkpoint immunotherapy resistance. 1,88Specifically, tumor cells can develop acquired resistance through loss of surface expression of MHC-I molecules via mutations in the B2M light chain (Figure 1b). 1,88Without B2M, the MHC-I molecules cannot be folded and transported to the surface of the cells in order to present the antigen; thus, CD8 + T cells will not be able to recognize the antigen and clear out the tumor cell. 88In addition, mutations in genes encoding transporter proteins, such as transporters associated with antigen presentation 1/2 (TAP) 1/ 2, which are involved in the process of importing antigen peptides into the endoplasmic reticulum, inhibit the formation of MHC-I-peptide complexes, thereby preventing the antigen processing and presentation machinery to proceed (Figure 1b). 88ogically, the presence of targetable tumor antigens is a critical factor contributing to the ability of T cells to mount an immune response and to the ability to gain clinical benefits with ICI therapy. 89These tumor antigens can be generated through genetic diversity, missense, and silent mutations, insertions, deletions, as well as copy number gains and losses. 89For example, cancers with MMR-D as a result of loss of function of certain genes (MLH-1, PMS-2, MSH-2, and MSH-6) involved in the DNA repair pathway and those that have MSI-H are examples of cancers with TMB-H. 40Both MSI-H and MMR-D induce frameshift mutations allowing for neoantigen formation. 38,40he number of all the somatic mutations per megabase (Mb) in the genome of a tumor cell, also known as TMB, seems to be the driver generating the immunogenic neoantigens presented by the MHC complex. 89,902][93] As such, melanoma and NSCLC are among the malignancies with TMB-H and higher OR to anti-PD-1 therapy. 38,94Nonetheless, only 45% of the patients with TMB-H responded to ICI therapy, indicating the existence of other mechanisms promoting primary resistance in these cases. 91,95hereas high ORR and OS are associated with tumors with TMB-H, cancers categorized as low TMB (TMB-L), defined as ≤5 mutations per Mb, 38,91 such as pancreatic cancer and triple-negative breast cancer (TNBC), have a low probability of response to ICIs and manifest as primary or acquired resistance. 38,91,96,97These observations were highlighted in a study that analyzed patients across several solid tumors, excluding NSCLC and melanoma, who had TMB assessment and received ICI therapy.The study revealed that only a low percentage of patients (9%) harboring TMB-L tumors had CR/ PR; conversely, 47% of the patients with TMB-H had CR/PR. 95

Presence of alternative inhibitory immune checkpoints
1][102] Although the specific mechanisms through which VISTA exerts immune inhibition are still unclear, its role in acquired resistance is being elucidated.A study of metastatic melanoma patients treated with ipilimumab found that the majority of patients (12/18) had biopsies with significantly higher densities of VISTA + lymphocytes during disease progression compared to pre-treatment. 98,103nterestingly, another study that analyzed tumor tissue biopsies from prostate cancer patients found that suppressive macrophages express either VISTA (26.5%) or PD-L1 (29.4%), but rarely both markers (2%). 85,98The presence of distinct macrophages suggests that these subsets may compensate for each other during ICI therapy. 85,98Overall, there is evidence that VISTA may be a compensatory inhibitory pathway that results in acquired resistance to currently available ICI therapy. 103AG-3 is expressed on activated T cells, natural killer (NK) cells, B cells, and dendritic cells (DCs), and currently, the only known ligand for LAG-3 is the MHC-II molecule.100 LAG-3/ MHC-II high-affinity interaction blocks proper TCR signaling, resulting in hampered T cell functions.104 Data showed that MHC-II expression in tumor cells is associated with increased response to anti-PD-1 therapy.105 A follow-up study demonstrated that MHC-II expression in tumors is associated with an increased infiltration of LAG-3+ TILS.100,106 Notably, LAG-3 expression was higher in specimens from patients who initially responded to PD-1 therapy but eventually progressed.107 Additionally, LAG-3 signaling can promote Treg populations through the release of immunosuppressive cytokines like transforming growth factor beta (TGF-β) and IL-10.104 Collectively, the growing body of data suggests that LAG-3 upregulation is associated with primary and acquired resistance in different cancer conditions.104,108 The immune checkpoint molecule TIM-3 is expressed in activated human T cells, NK cells, and monocytes.100 High TIM-3 expression in several syngeneic models has been associated with acquired resistance toward PD-1 blockade.[109][110][111] For example, Koyama et al. correlated acquired resistance after anti-PD-1 therapy with TIM-3 upregulation in a lung adenocarcinoma murine model. 110The same TIM-3 upregulation was observed in lung cancer patients who had PD following PD-1 blockade.Furthermore, TIM-3 expression was higher in the relapsed NSCLC patients when compared to those who were not treated with ICIs.110 Additionally, clinical data support that TIM-3 is universally co-expressed with PD-1 in TILs, enabling a more exhausted T cell phenotype.100,112 Indeed, a study associated the co-expression of PD-1 and TIM-3 with acquired resistance to anti-PD-1 therapy in NSCLC patients. 109TIGIT expression is restricted to CTLs, Th, Tregs, and NK cells.113,114 TIGIT's inhibitory signaling occurs upon interaction with its main ligand CD155 (PVR), expressed on tumor-infiltrating myeloid cells and tumor cells.114 Since TIGIT binds to CD155 with a higher affinity than its competitive ligand CD226, TIGIT/CD155 interaction disrupts CD226 homodimerization, inhibiting CD226mediated T cell activation. 113,114The TIGIT/CD155 complex can also reduce TCR-expression and TCR signaling.114 A study of melanoma correlated high CD155 and TIGIT expression with PD-1 and/or CTLA-4 primary and acquired resistance in patients with non-inflamed tumors and inflamed tumors with TMB-H.115 In addition, a study using a preclinical model of pancreatic adenocarcinoma (PDAC) supported the hypothesis that TIGIT blockade may overcome preexisting or acquired resistance to CD40a/PD-1 therapy.116

Immunosuppressive signaling
Tumor-cell extrinsic factors such as immunosuppressive cells, including Tregs and tumor-associated macrophages (TAMs), and inhibitory cytokines, such as TGF-β, also contribute to primary and/or acquired resistance in cancer (Figure 1d). 1,3In physiological conditions, the main function of Tregs is to maintain immune homeostasis; however, cancer exploits Treg cellular mechanisms to restrain the effector function of immunocompetent cells. 117Tregs can induce CTLA-4-mediated suppression of APCs, compete for IL-2 binding, produce antiinflammatory cytokines, and express immune inhibitory molecules such as TIGIT, PD-1, TIM-3, and LAG-3. 117Indeed, a study in NSCLC, gastric cancer and melanoma have suggested the involvement of Tregs in resistance to anti-PD-1 immunotherapy. 117This study demonstrated that the balance between PD-1 + CD8 + T cells and PD-1 + Tregs in the TME can better predict the outcome of PD-1 therapy than PD-L1 tumor tissue expression or TMB. 117GF-β is a pleiotropic cytokine that plays a key role in maintaining immune tolerance, yet is also involved in tumor evasion and immunotherapy resistance. 118,119A plethora of studies on solid tumors have correlated TGF-β signaling in the TME with different mechanisms underlying ICI resistance. 118,119For example, TGF-β can suppress TILs and at the same time induce high expression of PD-1 and PD-L1 in T cells and tumor cells, respectively. 118Additionally, a clinical study showed that high TGF-β gene signature can predict the failure of ICIs in gynecological cancer patients. 119The role of TGF-β in promoting primary resistance is yet to be understood; however, inhibition of TGF-β in a murine model refractory to anti-PD-1 improved antitumor response and survival benefits. 118,120,121etabolic modulators such as indoleamine 2,3-dioxygenase 1 (IDO) and adenosine can also play a role in fostering immunosuppressive conditions in the TME that result in immunotherapy resistance (Figure 1d). 104,122,123IDO is an intracellular enzyme that catalyzes the reaction that converts tryptophan into kynurenine under normal physiological conditions.It is expressed only in select tissues (mucosal tissues, placenta, eye, and pancreas) and by a small population of immune cells (DCs and eosinophils). 29,122However, data confirmed IDO upregulation in CRC, breast cancer, prostate cancer, and esophageal cancer. 29As such, expanding preclinical and clinical data correlated IDO upregulation with suppression of T cell function and ICI resistance. 29,122,124denosine accumulates in the TME through the conversion of hydrolyzed forms of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) or adenosine monophosphate (AMP), and then into adenosine by ectonucleotidases (CD39 and CD73), which are overexpressed in different cancer types. 123High levels of adenosine in the TME may also be due to mutations or hypoxic conditions that decrease the reduction of this metabolite (Figure 1d). 123Another source of adenosine in the TME comes from nicotinamide adenine dinucleotide (NAD + ) converted to AMP by CD38, a molecule that is upregulated by tumor cells and identified as another mechanism of resistance to PD-1 and PD-L1 checkpoint blockade (Figure 1d). 123

Strategies to overcome associated mechanisms contributing to immunotherapy resistance
As described above, response to ICI is contingent on the presence, abundance, and activity of tumor-reactive T cells.Treatments to induce effector T cell activation, infiltration, and function, therefore, are strategies that can be combined with ICI to enhance antitumor response or used after ICI therapy failure (Table 1).

Overcoming deficiencies in T cell priming
Lack of tumor antigen, defective antigen processing and presentation, and insufficient T cell-DC interaction all contribute to deficient T cell priming and activation that undermines the effect of immunotherapy.Anticancer treatments that induce immunogenic cell death (ICD), such as oncolytic viruses, radiotherapy, and certain types of chemotherapy and small-molecule inhibitors, can bridge this gap.Tumor cells undergoing ICD release antigens and damage-associated molecular patterns (DAMPs), allowing for the recruitment and maturation of APCs, the presentation of targetable antigens to effector T cells, and subsequent induction of antitumor immune responses and immune memory (Figure 2a). 128Furthermore, chemotherapy and radiotherapy can upregulate the antigen processing machinery, promote the expression of MHC class I, and elicit the expression of death receptors, rendering the tumor cells that survived the treatment more susceptible to immune attack (Figure 2a). 129,130][136] Most inducers of ICD, such as CAR T cells, are not "targeted," at least not to the same degree as checkpoint blockade; therefore, future studies on the immunotherapy field should focus on developing a consensus on the importance of defining a more generalized approach.Ongoing clinical trials are investigating the safety and the effect of combining ICD-inducers with ICI therapy, including in cancers that are refractory to checkpoint blockade (NCT03474497; Table 1).

Increasing the number of tumor-reactive T cells
Achieving high numbers of infiltrating tumor-reactive T cells is crucial for ICI response.The most direct way to increase the number of effector cells is via adoptive cell transfer (ACT) using TILs, TCR-engineered T cells, or chimeric antigen receptor (CAR)-engineered T cells.ACT with TILs involves isolating lymphocytes from resected tumors, expanding the cells ex vivo, and selecting for tumor-reactivity before reinfusing the TILs into patients. 137,138In a clinical study, TIL therapy was shown to induce durable responses in patients with metastatic melanoma.Notably, 11 patients enrolled in NSCLC: non-small cell lung carcinoma, RCC: renal cell carcinoma, HNSCC: head and neck squamous cell carcinoma, SCLC: small cell lung carcinoma, MCC: Merkel cell carcinoma, PLK1: polo-like kinase 1, UC: urothelial carcinoma, HC: hepatocellular carcinoma, MSI: microsatellite instability, MMR-D: mismatch repair deficiency, AGOC: advanced gastro-esophageal carcinoma.
the study had failed CTLA-4 treatment.Of the 11, five patients experienced complete regression while two experienced partial regression after TIL ACT. 137,138TCR T cells are peripheral T cells that are engineered to express tumor antigen-specific TCRs that are modified for improved expression and function. 139As such, TCR T cells rely on antigen presentation via MHC molecules but can be generated to target antigenic peptides of intracellular and extracellular origins.1][142] Preclinical studies combining NY-ESO-1 TCR T cells with ICI demonstrated improved antitumor activity related to increased T cell efficacy (Figure 2b). 143,144However, in a pilot trial, the combination of NY-ESO-1 TCR T cell and NY-ESO-1 DC vaccine resulted in transient antitumor response and the addition of ipilimumab to the combination did not improve clinical benefit, 145 indicating that optimization may be warranted.Clinical trials evaluating the efficacy of TCR T cells with anti-PD-1 or anti-PD-L1 are currently underway. 146CAR T cells express receptors composed of an antibody-derived single-chain variable fragment for antigen recognition fused to an intracellular signaling domain derived from T cell signaling proteins. 147ontrary to TCR T cells, CAR T cells are MHC-independent and can only target cell surface antigens (Figure 2b).Advancements in this ACT platform have led to six approved CAR T cell therapies for hematological cancers.In solid tumors, however, the efficacy of CAR T cells is severely limited partly due to the immunosuppressive environment that the adoptively transferred cells encounter. 148Hence, a great deal of effort is being exerted on studying the application of CAR T cells with ICI.Furthermore, the role of checkpoint blockade in ACT efficacy is paramount; thus, autologous T cells and CAR T cells with knocked out or disrupted PD-1 are being developed and evaluated. 149,150ancer vaccines can amplify a preexisting response by expanding tumor-specific T cell populations, broadening the T cell repertoire, and promoting the transport of T cells to tumor lesions (Figure 2c). 151,152Cancer vaccines are typically composed of soluble tumor antigens (e.g., oncoviral, oncofetal, cancer-testis, or neoantigens), formulation (e.g., peptide, nucleic acid, or whole tumor), delivery vehicle (e.g., liposome, cell-based, or viral-based), and an immune adjuvant (e.g., CD40 agonist, TLR agonist, or GM-CSF).Although monotherapy activity of cancer vaccines has so far been limited, its ability to sensitize host immunity to the tumor can complement ICI therapy, and this prospective synergistic activity is the focus of several clinical trials 153 (Table 1).The phase 1 Lipo-MERIT Trial (NCT02410733) aimed to evaluate the efficacy and safety of a tetravalent RNA vaccine (targeting the tumor antigens NY-ESO-1, MAGE-A3, tyrosinase, and TPTE) with or without PD-1 blockade in metastatic melanoma. 154artial responses were observed in both vaccine monotherapy (12%) and vaccine plus anti-PD-1 treatment (35.3%).Notably, patients with disease that progressed under ICI treatment achieved partial responses with the monotherapy or the combination.Furthermore, the response was associated with the induction of T cells specific to at least one vaccine-targeted antigen and the formation of memory T cells.The data suggest that cancer vaccines may be a strategy to overcome ICI resistance by synergizing with ICI in ICI-experienced tumors.
Immunostimulatory cytokines may also be key combination partners for ICI.One example is IL-12, which is a proinflammatory cytokine produced by APCs that promotes Th1 polarization, enhances effector cell recruitment, and boosts CD8 + T cell and NK cell cytolytic functions (Figure 2d). 155- 157Intratumoral administration of IL-12 in conjunction with CTLA-4 blockade has been shown to act synergistically to eradicate murine glioblastoma. 158Moreover, in murine tumor models resistant to anti-PD-1/anti-PD-L1 therapy, tumor-targeted NHS-IL12 (IL-12 fused to an antibody that binds exposed DNA commonly found in necrotic tumors) when administered with a histone deacetylase inhibitor potentiated CD8 + T cell-dependent antitumor activity and provided survival benefit. 33However, a phase 1b study evaluating the combination of NHS-IL12 and avelumab was discontinued due to lack of efficacy (NCT02994953).Several active studies that involve NHS-IL12 with anti-PD-L1/anti-TGF-β fusion protein are ongoing but have yet to post results (NCT04287868, NCT04303117).
0][161] In colon and prostate murine tumor models, the dual blockade of CTLA-4 and PD-L1 in combination with recombinant IL-15 (rIL-15) treatment resulted in tumor growth suppression associated with cytotoxic T cell activity, IFNγ secretion, and inhibition of Treg functions. 162,163Based on these findings, a phase 1 trial has been designed to test the safety of combining rIL-15 with nivolumab and ipilimumab in patients with refractory cancers (NCT03388632). 160Due to the short lifespan of rIL-15 in vivo, N803, an IL-15 superagonist composed of IL-15 mutant (IL-15N72D) complexed to a dimeric sushi domain of IL15Rα (IL-15 RαSu) and fused to an IgG-Fc fragment, was developed. 161,164The combination of N803 and ICI has been the focus of several preclinical and clinical studies.In syngeneic breast and colon murine tumor models, the combination of N803 and PD-L1 blockade promoted the activation, proliferation, and cytotoxicity of CD8 + T cells and NK cells, resulting in decreased tumor burden (Figure 2d). 165Meanwhile, in an oral squamous cell carcinoma model, N803 plus PD-1 blockade had moderate therapeutic efficacy that was further enhanced with the adoptive transfer of a PD-L1-targeted CAR-NK. 166In the clinic, a completed phase 1/phase 2 study in NSCLC patients demonstrated that N803 and nivolumab were well-tolerated. 167Furthermore, six (29%) of the 21 patients enrolled in the study achieved an objective response.Eleven of the 21 patients had relapsed after PD-1 blockade therapy and, of the 11, three (27%) had partial responses and seven (64%) had stable disease.Similarly, an ongoing phase 2b aims to evaluate the safety and efficacy of N803 plus ICI in patients who have progressed after PD-1/PD-L1 treatment (NCT03228667; Table 1).In this trial, patients with disease progression with N803 + ICI roll over to a new cohort and receive N803, PD-1/PD-L1 checkpoint inhibitor, and PD-L1-targeted CAR-NK.
The pathophysiological properties of tumors result in physical barriers that prevent the infiltration of effector cells.One impediment is the tumor vasculature, which is characterized by abnormal endothelial cells, chaotic vessel growth, leakiness, and erratic blood flow. 168Tumor vascular endothelial cells also express the death ligand FasL, killing extravasating CD8 + T cells while sparing Tregs that are not as sensitive to Fas-mediated apoptosis. 169urthermore, VEGF, the critical driver of angiogenesis, promotes an immunosuppressive ecosystem in the TME. 170The first anti-angiogenic drug, bevacizumab, an anti-VEGFmAb, was initially approved in 2004 as a treatment for colorectal cancer in combination with chemotherapy. 171,172Since then, research has been conducted to improve patient response to bevacizumab and other antiangiogenic drugs, mainly monoclonal antibodies and small-molecule tyrosine kinase inhibitors that target VEGF/VEGFR and other angiogenic pathways, in combination with other anti-cancer agents. 1735][176] The combination of ICI with antiangiogenic agents has also achieved success in the clinic.For instance, the openlabel, randomized phase 3 IMbrave150 (NCT03434379) study in patients with unresectable hepatocellular carcinoma showed that atezolizumab, an anti-PD-L1 antibody, with bevacizumab resulted in superior overall and progression-free survival outcomes than standard-of-care sorafenib, a multikinase inhibitor. 177,178This trial informed the FDA approval of this combination in hepatocellular carcinoma in 2020. 179In addition, a phase 1b clinical trial (NCT02715531) demonstrated that in patients with unresectable hepatocellular carcinoma, atezolizumab plus bevacizumab significantly prolonged progression-free survival compared with atezolizumab monotherapy. 180Collectively, these studies show that the combination of ICI with an antiangiogenic agent results in improved clinical benefit when compared to monotherapy with either treatment.
The extracellular matrix is another physical barrier that effector immune cells must overcome to effectively attack tumor cells.In addition to regulating the migration of T cells, collagen produced by cancer-associated fibroblasts, macrophages, and tumor cells can impair immune activity through interactions with leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1). 1813][184] An experimental antibody, NC410, that competes with LAIR-1 for collagenbinding is currently being evaluated in a phase 1 study in patients with advanced and metastatic solid tumors, such as ovarian, gastric, and colorectal cancer (NCT04408599).In murine EMT6 breast and MC38 colon cancer models, the combination of NC410 and an anti-PD-L1/anti-TGF-β fusion protein antibody (bintrafusp alfa, formerly M7824) improved antitumor activity by remodeling the collagen matrix, enhancing T cell tumor infiltration, and skewing the tumor-associated macrophages from the immunosuppressive M2 phenotype to immune-favorable M1 (Figure 2f). 32This preclinical study underscores the valuable contribution of targeting the extracellular matrix in facilitating ICI therapy.

Repolarization of the immunosuppressive microenvironment
The immunosuppressive tumor microenvironment is comprised of cellular components and soluble factors that promote tumor progression and contribute to immune resistance.The abundance of myeloid-derived suppressor cells (MDSCs), Tregs, TAMs, and other immunosuppressive cells in the peripheral blood or in the tumor lesion has been associated with poor prognosis in cancer patients. 185Immunosuppressive cells, together with tumor cells and stromal cells, can produce inhibitory cytokines (e.g., IL-10 and TGF-b) and factors (e.g., arginase, IDO, and collagen) and express checkpoint ligands to dampen the immune response. 185,186everal immuno-oncology agents that deplete or reprogram immunosuppressive populations by targeting markers overexpressed on those cells are under development and have the potential to synergize with ICIs.8][189] A phase 1 trial studying the combination of DS-8273a and nivolumab has recently been completed, but results are yet to be published (NCT02983006).Likewise, targeting colony stimulating factor-1 receptor (CSF-1 R), which regulates monocyte migration, proliferation, and differentiation into TAMs, has been shown to reduce TAM populations (Figure 2g). 190The safety and efficacy of axatilimab, an anti-CSF1-R antibody, in combination with nivolumab in patients with refractory/relapsed classical Hodgkin lymphoma is the subject of an ongoing phase 2 trial (NCT05723055, Table 1).Targeting MARCO, which is expressed on immunosuppressive M2-like TAMs, reprogrammed the macrophages into a pro-inflammatory phenotype and enhanced the antitumor efficacy of anti-CTLA-4 in melanoma and colon carcinoma models. 1916][197] A study investigating the safety and efficacy of another anti-GITR mAb, REGN6569, plus PD-1 blockade with cemiplimab is underway with results pending (NCT04465487).However, based on what is currently known, additional work will be required to translate the benefits of anti-GITR and ICI combination into an effective therapy. 198locking the recruitment and migration of immunosuppressive cells can also effectively repolarize the TME and sensitize tumors to ICI.0][201][202] SX-682, a small molecule dual inhibitor of CXCR1 and CXCR2, is currently being investigated in combination with anti-PD-1 (nivolumab, pembrolizumab, or tislelizumab) in patients with colorectal cancer (NCT04599140), melanoma (NCT03161431; Table 1), pancreatic cancer (NCT05604560), and nonsmall cell lung cancer (NCT05570825).
Lastly, another potential strategy to re-invigorate effector cells in tandem with ICIs is to target immunosuppressive factors in the TME.IDO is an enzyme involved in tryptophan catabolism, converting tryptophan to kynurenine.In cancer settings, the depletion of tryptophan and accumulation of kynurenine promotes Treg activation and inhibits NK and CD8+ T cell activation, thereby resulting in immunosuppression (Figure 2g). 203,204The IDO inhibitor, epacadostat, showed promising anti-tumor activity when combined with pembrolizumab and nivolumab in phase 1/2 trials. 205However, a phase 3 study of epacadostat plus pembrolizumab did not improve progression-free or overall survival in patients with unresectable or metastatic melanoma. 205,206Another metabolite that accumulates in the TME and plays an important role in immunosuppression is extracellular adenosine (eADO).The adenosine pathway ultimately results in the conversion of extracellular ATP to eADO via ectonucleotidases CD39 and CD73, which are overexpressed in tumors. 207Ligation of eADO to adenosine receptors (A2 A and/or A2 B ) on effector cells results in the reduction in CD8 + T cell function, differentiation of naïve CD4 + T cells to Treg cells, and decreased NK proliferation, survival, and function. 123Immuno-oncology agents targeting CD39, CD73, and adenosine and their potential combinations have been reviewed recently by Zahavi and Hodge (Figure 2h). 123Cytokines that support the immunosuppressive milieu can also be targeted to enhance ICI activity.TGF-β is expressed by tumor cells, stromal cells, and immunosuppressive cells that can attenuate CD8 + T cell function, 208,209 inhibit CD8 + T cell infiltration, 210 expand Tregs, 211 and polarize neutrophils into pro-tumor populations. 212The value of simultaneous blockade of PD-1/PD-L1 axis and TGF-β is underscored by the development of bintrafusp alfa, which is a fusion antibody composed of an anti-PD-L1 antibody fused to the extracellular domain of human TGF-β receptor II (Figure 2g).In preclinical studies, monotherapy with bintrafusp alfa was shown to effectively trap TGF-β and bind PD-L1, resulting in decreased tumor burden and prolonged survival. 213,214Furthermore, treatment with bintrafusp alfa had superior anti-tumor activity than treatment with either anti-PD-L1 or TGF-β, highlighting the importance of cotargeting these two inhibitory molecules.A recent publication by Gameiro et al. reviews past and current clinical trials involving bintrafusp alfa. 215

Conclusion
To date, the benefits of cancer immunotherapy are limited to certain cancer types, mainly because of the heterogenicity intrinsic to different tumor types and to the mechanisms promoting primary and acquired resistance.Efforts made in the field to overcome CBR-related unresponsiveness to ICI therapy have been focussed on overcoming T cell-related deficiencies and/or suppressing immunosuppressive populations in the TME.However, additional effort is needed to close the gap in knowledge in identifying additional checkpoints that may be abrogating the effectiveness of ICI therapy as well as unknown mechanisms underlying CBR.More detailed guidelines are needed as well to follow mixed responses after ICI therapy and to define primary and acquired resistance more accurately, not only in the setting of a clinical trial but also in the real-world after agent approval.

Disclosure statement
No potential conflict of interest was reported by the author(s).

Figure 1 .
Figure 1.Mechanism associated to ICI resistance.The TME represents a complex interaction between tumor cells, immune cells, and inhibitory cytokines leading to a plethora of mechanisms associated to ICI resistance.(a) Defects in T cells effector function and their exclusion from the TME can occur as a result of dysregulation of MAPK/ERK cell signaling pathways in tumor cells inducing the production of VEGF and IL-8, having a net inhibitory effect on the recruitment of T cells and defects in T-cell activation and differentiation.Due to the crosstalk between the MAPK and the PI3K signaling pathways, an oncogenic mutation in MAPK pathway or a loss of PTEN expression can cause the enhancement of PI3K-AKT signaling as well.Similarly, a mutation or silencing of β-catenin or tumor suppressor wnt protein, results in dysregulation of the WNT/β-catenin pathway, promoting aberrant signaling of b -catenin contributing to the absence of T cell expression signature.Mutations in the IFN-g receptor and defects on the IRF1 or JAK1/2 genes can contribute to T cell desensitization and consequently promote acquired resistance to ICIs.Specifically, mutations of PD-L1, PD-L2, and JAK2 genes resulting from the amplification of the locus that contains these genes promote the formation of the PD-1/TCR inhibitory microcluster, which results in the inhibition of T cell activation.(b) Another resistance mechanism is developed after defects in the APM specifically due to mutations of B2M and TAP proteins.These mutations make the antigen unable to reach the tumor cell's surface and be recognized and cleared out by CD8 + T cells.(c) the interaction between T cells, tumor cells, APCs, and immunosuppressive cells (MDSCs) throughout immune checkpoints such as VISTA, LAG-3, TIGIT, and TIM-3, triggers and inhibitory signal causing the exhaustion of T cells, blockage of TCR signaling, and decreasing T cell activation and TCR expression.(d) Immunosuppressive signaling also contributes to primary and/or acquired resistance in cancer.Tregs can restrain the effector function of immunocompetent cells by inducing checkpoint-mediated suppression (CTLA-4, PD-1, TIGIT, TIM-3, and LAG-3), competing for IL-2 binding, or by producing anti-inflammatory cytokines.As such, TGF-b is a pleiotropic cytokine involved in tumor evasion and immunotherapy resistance.The upregulation of the metabolic modulator IDO within the TME allows the depletion of tryptophan, resulting in the decrease of T cells.Another metabolic modulator associated with T cell function suppression and ICI resistance is adenosine.Several ectonucleotidases (CD39 and CD73) catalyze the conversion of ADP or AMP to adenosine or NAD + to AMP (CD38) causing the upregulation of adenosine in the tumor milieu.TME: tumor microenvironment; MAPK/ERK: mitogen-activated protein kinase/extracellular signal-regulated kinase; VEGF: vascular endothelial growth factor; IL-8: interleukin-8; PTEN: phosphatase and tensin homolog deleted on chromosome 10; PI3K-AKT: phosphoinositide-3-kinase -protein kinase B/Akt; WNT/β-catenin: wingless-related integration site/ b-catenin; IRF1: interferon regulatory factor 1; JAK1/2: Janus kinase 1/2; PD-L1/L2: program cell-death ligand 1/ligand 2; PD-1/TCR inhibitory microcluster: program cell-death 1/T cell receptor inhibitory microcluster; APM: antigen presenting machinery; β2 M; β2-microglobulin; TAP:transporter associated with antigen presentation; APC: antigen presenting cells; MDSCs: myeloid-derived suppressor cells; VISTA: V-domain ig suppressor of T cell activation; LAG-3: lymphocyte activation gene 3; TIGIT: T cell ImmunoGlobulin and ImmunoTyrosine inhibitory motif (ITIM) domain; TIM-3: T cell immunoglobulin and mucin-3; IDO: indoleamine 2,3-dioxygenase 1; CTLA-4:cytotoxic T lymphocyte antigen-4; TGF-β: transforming growth factor beta ; ADP: adenosine triphosphate; AMP: adenosine monophosphate; NAD + : nicotinamide adenine dinucleotide.
Figure 1.Mechanism associated to ICI resistance.The TME represents a complex interaction between tumor cells, immune cells, and inhibitory cytokines leading to a plethora of mechanisms associated to ICI resistance.(a) Defects in T cells effector function and their exclusion from the TME can occur as a result of dysregulation of MAPK/ERK cell signaling pathways in tumor cells inducing the production of VEGF and IL-8, having a net inhibitory effect on the recruitment of T cells and defects in T-cell activation and differentiation.Due to the crosstalk between the MAPK and the PI3K signaling pathways, an oncogenic mutation in MAPK pathway or a loss of PTEN expression can cause the enhancement of PI3K-AKT signaling as well.Similarly, a mutation or silencing of β-catenin or tumor suppressor wnt protein, results in dysregulation of the WNT/β-catenin pathway, promoting aberrant signaling of b -catenin contributing to the absence of T cell expression signature.Mutations in the IFN-g receptor and defects on the IRF1 or JAK1/2 genes can contribute to T cell desensitization and consequently promote acquired resistance to ICIs.Specifically, mutations of PD-L1, PD-L2, and JAK2 genes resulting from the amplification of the locus that contains these genes promote the formation of the PD-1/TCR inhibitory microcluster, which results in the inhibition of T cell activation.(b) Another resistance mechanism is developed after defects in the APM specifically due to mutations of B2M and TAP proteins.These mutations make the antigen unable to reach the tumor cell's surface and be recognized and cleared out by CD8 + T cells.(c) the interaction between T cells, tumor cells, APCs, and immunosuppressive cells (MDSCs) throughout immune checkpoints such as VISTA, LAG-3, TIGIT, and TIM-3, triggers and inhibitory signal causing the exhaustion of T cells, blockage of TCR signaling, and decreasing T cell activation and TCR expression.(d) Immunosuppressive signaling also contributes to primary and/or acquired resistance in cancer.Tregs can restrain the effector function of immunocompetent cells by inducing checkpoint-mediated suppression (CTLA-4, PD-1, TIGIT, TIM-3, and LAG-3), competing for IL-2 binding, or by producing anti-inflammatory cytokines.As such, TGF-b is a pleiotropic cytokine involved in tumor evasion and immunotherapy resistance.The upregulation of the metabolic modulator IDO within the TME allows the depletion of tryptophan, resulting in the decrease of T cells.Another metabolic modulator associated with T cell function suppression and ICI resistance is adenosine.Several ectonucleotidases (CD39 and CD73) catalyze the conversion of ADP or AMP to adenosine or NAD + to AMP (CD38) causing the upregulation of adenosine in the tumor milieu.TME: tumor microenvironment; MAPK/ERK: mitogen-activated protein kinase/extracellular signal-regulated kinase; VEGF: vascular endothelial growth factor; IL-8: interleukin-8; PTEN: phosphatase and tensin homolog deleted on chromosome 10; PI3K-AKT: phosphoinositide-3-kinase -protein kinase B/Akt; WNT/β-catenin: wingless-related integration site/ b-catenin; IRF1: interferon regulatory factor 1; JAK1/2: Janus kinase 1/2; PD-L1/L2: program cell-death ligand 1/ligand 2; PD-1/TCR inhibitory microcluster: program cell-death 1/T cell receptor inhibitory microcluster; APM: antigen presenting machinery; β2 M; β2-microglobulin; TAP:transporter associated with antigen presentation; APC: antigen presenting cells; MDSCs: myeloid-derived suppressor cells; VISTA: V-domain ig suppressor of T cell activation; LAG-3: lymphocyte activation gene 3; TIGIT: T cell ImmunoGlobulin and ImmunoTyrosine inhibitory motif (ITIM) domain; TIM-3: T cell immunoglobulin and mucin-3; IDO: indoleamine 2,3-dioxygenase 1; CTLA-4:cytotoxic T lymphocyte antigen-4; TGF-β: transforming growth factor beta ; ADP: adenosine triphosphate; AMP: adenosine monophosphate; NAD + : nicotinamide adenine dinucleotide.

Figure 2 .
Figure 2. Strategies to overcome associated mechanisms contributing to immunotherapy resistance.(a) Chemotherapy, radiotherapy, and small molecule inhibitors are among the strategies used to overcome deficiencies in T-cell priming.These agents can induce ICD in tumor cells causing the release of antigens and DAMPs, allowing the recruitment and maturation of APCs and the presentation of targetable antigens to effector T cells.Other interventions are used to increase the number of tumorreactive T cells interacting in the TME, such as (b) ACT using TCR T cell or CAR T cell.TCR T directed against specific cancer antigens (testis antigen, NY-ESO-1) can recognize the antigen through MCH molecules and CAR T cells act in an MHC-independent manner targeting cell surface antigens.(c) the use of cancer vaccines is also used to expand tumor-specific T cell populations, broaden the T cell repertoire, and promote the transport of T cells to tumor lesions.Additionally, the use of (d) immunostimulatory cytokines such as NHS-IL-12 or N803 can promote the enhancement of effector cell recruitment and boost CD8 + T cell and NK cell cytolytic functions.(e) physical barriers also prevent the infiltration of effector T cells in the TME, and tumor vasculature represents one of these barriers.Therefore, VEGF targeting using small molecule inhibitors or mAbs represents an effective strategy to disrupt angiogenic pathways that are fostering the aberrant vasculature.(f) another physical barrier is the extracellular matrix that is composed mainly of collagen.Collagen is produced by TAMs, CAFs, and tumor cells and can impair immune activity through interactions with LAIR-1.Blockade of LAIR-1 combined with PD-1, and TGF-β blockade can increase M1 TAMs population, CD8 + T cell infiltration, while decreasing TGF-β and collagen.Figure adapted from J Clin Invest.2022;132 8 : e155148.https://doi.Org/10.1172/JCI155148.lastly, repolarization of the immunosuppressive microenvironment as an alternative strategy to overcome certain resistant mechanisms can be achieved by (g) the use of mAbs targeting CD33 and TRAIL-R2 receptors expressed in tumor cells were demonstrated to eliminate MDSCs.The use of anti-CFR-1 were demonstrated to eliminate M2 macrophages.Likewise, the depletion of tregs using anti-CD4, anti-CD25 or anti-GITR in combination with ICI have shown to improve CD8+ T cell activity.Another approach uses to decrease the

Table 1 .
Clinical trials recruiting patients with primary refractory or acquired secondary resistance to prior immune checkpoint treatments.